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Key Documents

S8140

Sigma-Aldrich

SSPE Buffer

for Northern and Southern blotting, powder blend

Synonyme(s) :

Saline-Sodium Phosphate-EDTA Buffer

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About This Item

Code UNSPSC :
41105319
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

200

Forme

powder blend

pH

7.2-7.6 (20 × in water, high purity, ambient)

Solubilité

water, high purity: 20 ×, clear, colorless

Activité étrangère

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

Température de stockage

room temp

Catégories apparentées

Description générale

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

Application


  • Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).

  • Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

Caractéristiques et avantages

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Composants

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures
Igor V. Kutyavin
Nucleic Acids Research, 28(2) (2000)
Microfluidic-based enzymatic on-chip labeling of miRNAs
Sonja Vorwek
New Biotechnology (2008)

Protocoles

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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