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P5998

Sigma-Aldrich

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse

~2 mg/mL, clone 35-206, purified immunoglobulin, buffered aqueous solution

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About This Item

Numéro MDL:
MFCD07370940
Code UNSPSC :
12352203
eCl@ss :
32160410
Nomenclature NACRES :
NA.44

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

35-206, monoclonal

Forme

buffered aqueous solution

Espèces réactives

human, rat, mouse

Concentration

~2 mg/mL

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.04-0.08 μg/mL using whole extract of HeLa cell line

Isotype

IgG2b

Numéro d'accès UniProt

P53350

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PLK1(5347)
mouse ... Plk1(18817)
rat ... Plk1(25515)

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Description générale

Monoclonal Anti- hPlk1 (Polo Like Kinase 1) (mouse IgG2b isotype) is derived from the 35-206 hybridoma produced by the fusion of mouse myeloma cells (PAI) and splenocytes from BALB/c mice immunized with human full length Plk1. Polo-like kinases (Plks) are a family of serine/threonine kinases. The Plk family is structurally and functionally related to the polo gene in Drosophila. Plk1 localizes during mitosis in centrosomes, kinetochores and in the central spindle.

Immunogène

human full length Plk1.

Application

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunofluorescence staining

Actions biochimiques/physiologiques

PLK1 is a serine-threonine kinase that is expressed in mitotic cells and cancers. This kinase regulates mitotic entry by modulating cdc2/cyclin B and is also involved in Nlp-mediated nucleation of microtubules .
Polo Like Kinase 1 (Plk1) has a role in tumor progression. Plk1 is a target of the DNA damage checkpoint on the basis that its activity is inhibited by DNA damage in G2 and mitosis. Moreover, Plk1 was found to bind to the sequence-specific DNA binding domain of p53. This interaction between p53 and Plk1 inhibits the transcriptional activation and the proapoptotic functions of p53.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Association of spindle midzone particles with polo-like kinase 1 during meiosis in mouse and human oocytes
Otsuki J, et al.
Reproductive Biomedicine Online, 18(4), 522-528 (2009)
The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex
Cheng KY, et al.
The Embo Journal, 22(21), 5757-5768 (2003)
U Holtrich et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(5), 1736-1740 (1994-03-01)
We have identified the nucleotide sequence of the cDNA encoding the human counterpart of the mouse gene Plk (polo-like kinase). The sequence of the human gene, PLK, predicts a serine/threonine kinase of 603 aa. Expression of PLK mRNA appeared to
Martina Casenghi et al.
Developmental cell, 5(1), 113-125 (2003-07-11)
In animal cells, most microtubules are nucleated at centrosomes. At the onset of mitosis, centrosomes undergo a structural reorganization, termed maturation, which leads to increased microtubule nucleation activity. Centrosome maturation is regulated by several kinases, including Polo-like kinase 1 (Plk1).
Polo-like kinase 1 (Plk1) inhibits p53 function by physical interaction and phosphorylation
Ando K, et al.
The Journal of biological chemistry, 279(24), 25549-25561 (2004)
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