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P2545

Millipore

Protéine A-agarose

saline suspension

Synonyme(s) :

Protein A resin

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About This Item

Numéro MDL:
MFCD00165563
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Source biologique

Staphylococcus aureus

Forme

saline suspension

Ampleur du marquage

~2 mg per mL

Activation de la matrice

cyanogen bromide

Fixation de matrice

amino

Espaceur de matrice

1 atom

Capacité

≥15 mg/mL binding capacity (human IgG)

Température de stockage

2-8°C

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Description générale

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Application

Protein A−Agarose has been used in the immunoprecipitation
  • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
  • of prolyl hydroxylase domain 2 N27 cells
  • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Actions biochimiques/physiologiques

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Forme physique

Suspension in 0.5 M NaCl containing preservative

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

D J McCool et al.
The Biochemical journal, 302 ( Pt 1), 111-118 (1994-08-15)
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each
PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma
Zeleniak AE, et al.
Neoplasia, 20(1), 12-24 (2018)
D J McCool et al.
The Biochemical journal, 341 ( Pt 3), 593-600 (1999-07-27)
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to look for possible involvement of the chaperones calreticulin (CRT) and calnexin
Hsp90 Co-chaperone p23 contributes to dopaminergic mitochondrial stress via stabilization of PHD2: Implications for Parkinson?s disease
Rane A, et al.
Neurotoxicology, 65, 166-173 (2018)
Evaluation of a novel methacrylate-based protein a resin for the purification of immunoglobulins and Fc-fusion proteins
McCaw TR, et al.
Biotechnology Progress, 30(5), 1125-1136 (2014)
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