P1782

Protein kinase Cα isozyme human

≥70% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

• Synonyme(s) : PKCα
• Numéro MDL: MFCD00241710
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in baculovirus infected insect cells
• Niveau de qualité: 200
• Pureté: ≥70% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Activité spécifique: ≥250 units/μg protein
• Poids mol.: 80-81 kDa by SDS-PAGE
• Numéro d'accès UniProt: P17252
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PRKCA(5578)

Description

Actions biochimiques/physiologiques

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP₂) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca²⁺ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca²⁺-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

Définition de l'unité

One unit will transfer 1 pmol of phosphate from ATP to Histone H1 in 1 minute at pH 7.4 at 30 °C

Forme physique

Supplied as a buffered aqueous glycerol solution, containing Trizma buffer, NaCl, DTT and Glutathione.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)