O4264

Human Osteopontin

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Synonyme(s) : Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• Numéro MDL: MFCD03100698
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• product name: Osteopontin human, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in NSO cells
• Stérilité: sterile
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Poids mol.: 60-65 kDa by SDS-PAGE (reducing)
• Conditionnement: pkg of 50 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P10451
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• Informations sur le gène: human ... SPP1(6696)

Description

Description générale

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

Application

Anti-Osteopontin antibody produced in rabbit has been used for ELISA.

Actions biochimiques/physiologiques

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured by its ability to mediate 293 cell adhesion.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)