O3514

Bovine Osteopontin

from bovine milk, powder, suitable for cell culture

• Synonyme(s) : Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• Numéro MDL: MFCD03100698
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• product name: Osteopontin from bovine milk, lyophilized powder
• Source biologique: bovine milk
• Niveau de qualité: 100
• Stérilité: sterile
• Forme: lyophilized powder
• Poids mol.: ~60 kDa
• Conditionnement: pkg of 50 μg
• Technique(s): cell culture | mammalian: suitable
• Numéro d'accès UniProt: P31098; P31096
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• Informations sur le gène: cow ... SPP1(281499)

Description

Description générale

Osteopontin (OPN) is a multifunctional protein. It is released by macrophages, T cells and epithelial cells. It has 261 amino acid residues with a molecular mass of 29,283 Da. Osteopontin is also called as uropontin. Osteopontin from bovine milk is highly glycosylated and phosphorylated. SPP1 gene (secreted phosphoprotein 1) codes for osteopontin. This gene is located on human chromosome 4 and cow chromosome 6. It has seven exons and six introns.

Actions biochimiques/physiologiques

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity.

Osteopontin stimulates cell mediated responses, chemotaxis of inflammatory cells and anti-inflammatory responses. It binds to av-containing integrins and stimulate cell adhesion and migration. Osteopontin promotes Th1 type immunity.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured by its ability to mediate 293 cell adhesion.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)