Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

View All Documentation

N3162

Sigma-Aldrich

Anti-NBS1 (Nibrin) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-p95 Protein of the MRE11/RAD50 Complex

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Numéro MDL:
MFCD07784851
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 95 kDa (also recognizes 100 kDa band)

Espèces réactives

canine, human, rat, chimpanzee

Technique(s)

immunoprecipitation (IP): 5-10 μg
indirect immunofluorescence: 5-10 μg/mL using Hela cells, fixed with paraformaldehyde/triton
microarray: suitable
western blot: 1-2 μg/mL using MCF7 nuclear extracts

Numéro d'accès UniProt

O60934

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... NBN(4683)
mouse ... Nbn(27354)
rat ... Nbn(85482)

Description générale

NBS1 (Nibrin), also known as p95 protein of the meiotic recombination 11 homolog 1/DNA repair protein RAD50 (MRE11/RAD50) complex. NBS1 contains two domains found in the cell cycle checkpoint proteins forkhead-associated domain (FHA) and an adjacent breast cancer carboxyterminal domain (BRCT). NBS1 is one of the protein components of the double-strand break repair complex NBS1/MRE11/p50.
Nibrin (NBS1) is made up of 754 amino acids.

Immunogène

synthetic peptide corresponding to amino acids 692-706 of mouse NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue.

Application

Anti-NBS1 (Nibrin) antibody has been used:
  • in immunofluorence
  • in immunoblotting
  • in immunoprecipitation

Actions biochimiques/physiologiques

NBS1 (Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with Nijmegen breakage syndrome (NBS). The majority of NBS patients bear a five base pairs deletion that leads to truncated NBS1, called 657del5. p95/NBS1 (nibrin) deficiency abrogates the formation of the MRE11/RAD50 ionizing radiation-induced foci, revealing a molecular link between DSB repair and cell cycle checkpoints activated by DNA damage.
Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA.

Forme physique

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Adel Alblihy et al.
Biomedicines, 9(1) (2021-01-14)
Platinum resistance seriously impacts on the survival outcomes of patients with ovarian cancers. Platinum-induced DNA damage is processed through DNA repair. NBS1 is a key DNA repair protein. Here, we evaluated the role of NBS1 in ovarian cancers. NBS1 expression
Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome
Varon R, et al.
Cell, 93(3), 467-476 (1998)
VRK1 phosphorylates and protects NBS1 from ubiquitination and proteasomal degradation in response to DNA damage
Monsalve D M, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1863(4), 760-769 (2016)
Jana Suchánková et al.
Biology of the cell, 107(12), 440-454 (2015-10-21)
The DNA damage response is a fundamental, well-regulated process that occurs in the genome to recognise DNA lesions. Here, we studied kinetics of proteins involved in DNA repair pathways and their recruitment to DNA lesions during the cell cycle. In
Kelly Gray et al.
Circulation research, 116(5), 816-826 (2014-12-20)
DNA damage and the DNA damage response have been identified in human atherosclerosis, including in vascular smooth muscle cells (VSMCs). However, although double-stranded breaks (DSBs) are hypothesized to promote plaque progression and instability, in part, by promoting cell senescence, apoptosis
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique