05-616-C

Anti-Nibrin/Nbs1 Antibody, clone EE15 (Ascites Free)

clone EE15, from mouse

• Synonyme(s) : Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, p95 protein of the MRE11/RAD50 complex
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: EE15, monoclonal
• Espèces réactives: human
• Ne doit pas réagir avec: mouse
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_002476
• Numéro d'accès UniProt: O60934
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Nbn(27354)

Description

Description générale

Nibrin (UniProt O60934; also known as Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, p95 protein of the MRE11/RAD50 complex) is encoded by the NBN (also known as AT-V1, AT-V2, ATV, NBS, NBS1, P95) gene (Gene ID 4683) in human. Nibrin is a component of the MRN (MRE11/RAD50/NBN) complex involved in early steps of DNA double strand breaks (DSBs). The phosphorylation of H2AX pSer139 (γ-H2AX) found at the DNA-damaged sites, for example, is mediated by a direct interaction between Nibrin and the phosphorylated mediator of the DNA damage checkpoint 1 (MDC1). ATM, CtIP/RBBP8, Tip60, BRCA1, and SMC1 are also known interaction partners of Nibrin. Nibrin contains a fork-head associated (FHA) domain and two BRCA1 C-terminal (BRCT) tandem domains in its N-terminal region, followed by two MRE11-binding motifs and an ATM-binding motif in its C-terminal region. The Ser278 and Ser343 residues are known to be targeted for phosphorylation by ATM in response to ionizing-radiation (IR). NBN gene mutations are responsible for Nijmegen breakage syndrome (NBS), a disorder characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer.

Spécificité

Recognizes Nibrin/Nbs1, Mr 95kDa. A non-specific protein was also detected at Mr 33kDa in some lysates, but not Jurkat cell lysate.

Immunogène

Epitope: Amino acids 420-774.

GST fusion protein corresponding to residues 420-774 of human Nibrin/Nbs1.

Application

Anti-Nibrin/Nbs1 Antibody, clone EE15 (Ascites Free) is an antibody against Nibrin/Nbs1 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected Nibrin/Nbs1 in A431 cells.
Western Blotting Analysis: A representative lot detected a shift of Nbs apparent molecular weight in lysates from gamma-irradiated human cells (U2OS, IMR90, HEK293, BJAB) (Wu, X., et al. (2000). Nature. 405(6785):477-482).
Immunocytochemistry Analysis: A representative lot detected NBS1-positive nuclear foci by fluorescent immunocytochemistry (Wu, X., et al. (2000). Nature. 289(5476):11).
Immunoprecipitation Analysis: 4 μg of this antibody immunoprecipitated Nibrin/Nbs1 from 500 μg of Jurkat RIPA lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nibrin/Nbs1 in 10 µg of HeLa cell lysate.

Description de la cible

~95 kDa observed.

Liaison

Replaces: 05-616

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable