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Key Documents

MWND500

Sigma-Aldrich

Kit for Molecular Weights 14,000-500,000 Non-denaturing

Synonyme(s) :

protein markers, protein molecular weight standards, protein standards

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About This Item

Code UNSPSC :
41105337
Nomenclature NACRES :
NA.32

Forme

lyophilized powder

Température de stockage

−20°C

Application

Kit for Molecular Weights 14,000-500,000 Non-denaturing has been used as a standard for non-denaturing PAGE (polyacrylamide gel electrophoresis) analysis. It has also been used to calibrate columns for gel exclusion chromatography.

Conditionnement

The kit contains 5 vials of proteins (1 mg of each) having a molecular weight range of 14 - 545 kDa and a technical bulletin.

Proteins:
α-Lactalbumin from bovine milk
Carbonic Anhydrase from bovine erythrocytes
Albumin from chicken egg white
Albumin from bovine serum
Urease from Jack bean

Reconstitution

Reconstitute the contents of each vial of protein marker with 1ml of water, with the exception of Urease, Catalog Number U7752. Reconstitute the vial of Urease with 0.5 ml of water and mix until the protein is in solution. Then add 0.5 ml of glycerol to the Urease solution to stabilize the protein during freeze thaw cycles. Solutions may be stored at -20 °C for future use. It is recommended that repeated freezing and thawing be avoided. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2-3 uses. Immediately before use, thaw an aliquot of each prepared protein marker and dilute with an equal volume of sample buffer.

Pictogrammes

Health hazardExclamation mark

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

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