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G5262

Supelco

Glycéraldéhyde-3-phosphate déshydrogénase from rabbit muscle

standard for protein electrophoresis

Synonyme(s) :

D-Glycéraldéhyde 3-phosphate:NAD+ oxydoréductase (phosphorylante), GAPDH

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About This Item

Numéro CAS:
9001-50-7
Numéro de classification (Commission des enzymes):
1.2.1.12 (BRENDA, IUBMB)
Numéro CE :
232-609-4
Numéro MDL:
MFCD00131203
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

Qualité

for molecular biology

Niveau de qualité

200

Forme

powder

Poids mol.

~36 kDa

Conditionnement

vial of 5 mg

Technique(s)

electrophoresis: suitable

Température de stockage

2-8°C

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Catégories apparentées

Description générale

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport.

Application

GAPDH protein is suitable for use as a molecular weight marker and protein standard for molecular biology applications, including western blotting and mass spectometry.

Actions biochimiques/physiologiques

La glycéraldéhyde-3-phosphate déshydrogénase catalyse la conversion du glycéraldéhyde-3-phosphate en D-glycérate-1,3-bisphosphate dans le cadre de la voie de la glycolyse.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

P7170

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

C8302

4-Chloro-1-naphthol solution, For HRP detection on Western Blots

P7059

Solution tampon phosphate, 10× PBS for Western blots and IP

B5655

SIGMAFAST™ BCIP®/NBT, tablet

B6404

BCIP®/NBT solution, premixed

RPROB

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

W0138

Western Blocker™ Solution, for HRP detection systems

SDS7B2

Prestained Molecular Weight Marker, mol wt 26,600-180,000 Da

C1992

ColorBurst™ Marker, mol wt 8,000-220,000 Da

GERPN2209

ECL™ Western Blotting Detection Reagents, Cytiva RPN2209

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Ling Ji et al.
Molecular therapy. Nucleic acids, 19, 546-561 (2020-01-14)
Long non-coding RNAs (lncRNAs) are crucial molecules in tumorigenesis and tumor growth in various human cancers, including colorectal cancer (CRC). Studies have revealed that lncRNAs can regulate cellular processes in cancers by interacting with proteins, for example RNA-binding proteins (RBPs).
Kati Juuti-Uusitalo et al.
Investigative ophthalmology & visual science, 54(5), 3510-3519 (2013-05-21)
Aquaporins (AQPs), a family of transmembrane water channel proteins, are essential for allowing passive water transport through retinal pigmented epithelial (RPE) cells. Even though human native RPE cells and immortalized human RPEs have been shown to express AQPs, the expression
Craig Nicholls et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(33), 13308-13313 (2012-08-01)
Oxidative stress regulates telomere homeostasis and cellular aging by unclear mechanisms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a key mediator of many oxidative stress responses, involving GAPDH nuclear translocation and induction of cell death. We report here that GAPDH interacts with
J E Welch et al.
Journal of andrology, 21(2), 328-338 (2000-03-14)
Although the process of glycolysis is highly conserved in eukaryotes, several glycolytic enzymes have unique structural or functional features in spermatogenic cells. We previously identified and characterized the mouse complementary DNA (cDNA) and a gene for 1 of these enzymes
Xiaopeng Wang et al.
Molecular medicine reports, 23(2) (2020-12-11)
Acute progressive hypoxic respiratory failure caused by various predisposing factors is known as acute respiratory distress syndrome (ARDS). Although penehyclidine hydrochloride (PHC), an anticholinergic drug, is widely applied in clinical practice, the specific mechanisms underlying PHC in the treatment of
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