MTOX1300
Canine MDCKII Wild Type Cells
canine
Synonyme(s) :
MDCK II, MDCK-2, MDCKII-WT
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81
Produits recommandés
product name
Canine MDCKII Wild Type Cells,
Source biologique
canine
Niveau de qualité
Forme
liquid
Température de stockage
−196°C
Description générale
MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435).
Application
Canine MDCKII Wild Type Cells can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. See technical bulletin for detailed protocols.
Caractéristiques et avantages
MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron.
Qualité
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.
Stockage et stabilité
Upon receiving a shipment of frozen canine MDCKII wild type cells it is important the end user gives the shipment attention without delay. To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70 °C. Storage at –70 °C will result in loss of viability.
Informations légales
Exhibit 1: ADME/Tox cell lines licenseMDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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D Louvard
Proceedings of the National Academy of Sciences of the United States of America, 77(7), 4132-4136 (1980-07-01)
A dog kidney epithelial cell line (MDCK), grown in monolayer, displayed in vitro an asymmetric localization of surface proteins. Aminopeptidase [alpha-aminoacylpeptide hydrolase (microsomal), EC 3.4.11.2] was found only in the apical face whereas Na+, K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was
G C Hansson et al.
The EMBO journal, 5(3), 483-489 (1986-03-01)
The glycosphingolipids (GSLs) of two sublines of Madin-Darby canine kidney (MDCK) cells, an epithelial cell line, were characterized by t.l.c., antibody overlay and mass spectrometry. The major characteristic which distinguishes the two MDCK cell strains is their trans-epithelial electrical resistance
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