MDCK I - Madin-Darby canine kidney- is a subclone derived from the heterogeneous parent line MDCK (Madin-Darby canine kidney, Sigma Catalogue number. 85011435). MDCK-I cells, also known as "low passage MDCK" are reported to display a high electrical resistance (over 1000 Ohm/cm2) and form a monolayer with characteristics of tight epithelia of the renal collecting duct. Grown on filters, the cells show a cuboidal polarised morphology and express microvillae-like projections on the apical surface. Cultures on glass possess junctions but no polarised or columnar morphology. MDCK-I cells display an adrenalin, vasopressin and prostoglandin E sensitive adenyl cyclase. Please note, these cells were mycoplasma erradicated prior to deposit at ECACC. Epithelial phenotype may be unstable. Incomplete harvesting at confluence may select for smooth-muscle phenotype this is important if cells being used for transepithelial resistance studies.
Application
Virus susceptibility: Influenza virus, vesicular stomatitis virus and many other viruses.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000cells/cm2 using 0.25% tryspin or trypsin/EDTA; 5% CO2; 37°C
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