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MP0040A

Sigma-Aldrich

LookOut® Mycoplasma qPCR Detection Kit

Synonyme(s) :

Quantiative PCR mycoplasma detection kit

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About This Item

Numéro CE :
200-664-3
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Utilisation

sufficient for 25 reactions

Niveau de qualité

200

Qualité

Not suitable for clinical diagnostic use.

Conditionnement

pkg of 1 kit

Conditions de stockage

dry at room temperature

Technique(s)

PCR: suitable

Application(s)

detection
microbiology

Température de stockage

2-8°C

Catégories apparentées

Description générale

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.

The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.

This kit already contains Taq polymerase in the Mycoplasma Mix.

Application

qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

Informations légales

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F

Point d'éclair (°C)

87 °C

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Protocoles

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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