10799050001

BM-Cyclin

lyophilized, active toward mycoplasma

• Synonyme(s) : antibiotic, minocycline, tiamulin
• Code UNSPSC : 12352207

Propriétés

• Forme: lyophilized
• Niveau de qualité: 100
• Conditionnement: pkg of 37.5 mg (for 2 x 2.5 l medium)
• Fabricant/nom de marque: Roche
• Spectre d'activité de l'antibiotique: mycoplasma
• Mode d’action: protein synthesis | interferes
• Température de stockage: 2-8°C

Description

Description générale

BM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis.

Spécificité

Sensitive Organisms: Mycoplasmas and bacteria

• BM Cyclin was found to effectively eliminate Acholesplasma laidlawii,
• Mycoplasma arginini,
• Mycoplasma hyorhinis, and
• Mycoplasma orale

from experimentally contaminated and chronically infected cell lines.
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.

Application

BM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects.

Conditionnement

Set of two different antibiotics

Forme physique

Vial 1 (Tiamulin-hydrogenfumarat): clear, colorless solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution

Notes préparatoires

Working concentration: 10μg/ml pleuromutilin derivative (BM-cyclin 1), 5μg/ml tetracycline derivative (BM-cyclin 2).
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1μg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

• BM-Cyclin 1: pleuromutilin derivative
• BM-Cyclin 2: tetracycline derivative

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302,H315,H319,H335
• Conseils de prudence: P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
• Classification des risques: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable