MAK457
Caspase-3 Assay Kit
Sufficient for 100 fluorometric tests
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Niveau de qualité
Entrée
cell lysate
tissue
Méthode de détection
fluorometric
Maladie(s) pertinente(s)
neurological disorders; cancer
Température de stockage
−20°C
Description générale
CPP32 is a gene encoding a member of the caspase family, crucial for both the initiation and execution phases of apoptosis, a fundamental process in various physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Human caspases are categorized into three functional groups: cytokine activation, apoptosis initiation, and apoptosis execution. Among these, Caspase 3, also known as CPP32, Apopain, Yama, and PARP cleavage protease, is extensively studied and regulates numerous cellular events. Its activity is modulated by various factors, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family. Caspase 3 undergoes self-proteolysis and/or cleavage by other proteases, resulting in its activation. It subsequently cleaves and activates other caspases and targets in cells. Variations in Caspase 3 levels are observed in cells with different lifespans and in diseases like Alzheimer′s. Caspase-3 serves as a vital biomarker in apoptosis assessment and aids in understanding its underlying mechanisms.
Application
The Caspase-3 Assay Kit may be used for:
- Apoptosis Inducer and Inhibitor Screening
- Cancer Research
- Neurological Research
- Drug Discovery
Caractéristiques et avantages
- Efficient Procedure: Simplify your workflow with our hassle-free process, requiring only the addition of a single working reagent directly to your cell or lysate samples, saving valuable time and effort.
- Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.
Adéquation
The kit is suitable for the quantitative determination of the apoptosis target caspase-3 activity and high throughput screening for apoptosis inducers and inhibitors in cell and tissue lysate.
Principe
The Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product. The fluorescence intensity (λEx = 400 nm/λEm = 490 nm) is proportional to the caspase-3 activity.
Autres remarques
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Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Dam. 1 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
188.6 °F
Point d'éclair (°C)
87 °C
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