MAK178
Enolase Activity Assay Kit
Sufficient for 100 Colorimetric or Fluorometric tests
Synonyme(s) :
Enolase Detection Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Méthode de détection
colorimetric
fluorometric
Maladie(s) pertinente(s)
immunological diseases; cardiovascular diseases
Température de stockage
−20°C
Informations sur le gène
human ... EBI4(387712) , ENO1(2023) , ENO2(2026)
mouse ... EBI4(226265) , ENO1(13806) , ENO2(13807)
rat ... EBI4(292138) , ENO1(24333) , ENO2(24334)
Catégories apparentées
Description générale
Enolase is a multifunctional glycolytic enzyme that catalyzes the conversion of D-2-phosphoglycerate to phospho(enol)pyruvate (PEP) and water. It can function as a plasminogen receptor in endothelial, epithelial, and hematopoietic cells and hence, may be involved in fibrinolytic and intravascular systems. It is also known to act as a heat shock protein, which may have implications in transcriptional and pathological functions. Enolase has been implicated in autoimmune and systemic diseases. Furthermore; serum neuron-specific enolase is known to function as a predictor of patient outcome post cardiac arrest. Thus enolase assays can be used for studying cellular functions like carbohydrate metabolism, transcription and other pathophysiological processes.
Application
Enolase Activity Assay Kit has been used to measure enolase activity.
Adéquation
Suitable for the detection of enolase activity in biological samples.
Principe
The Enolase activity is determined by a coupled enzyme assay in which D-2-phosphoglycerate is converted to PEP, resulting in the formation of an intermediate that reacts with a peroxidase substrate, generating a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product proportional to the enolase activity present. One milliunit of enolase is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 at 25 °C.
Composants de kit seuls
Réf. du produit
Description
- Enolase Assay Buffer
- Peroxidase Substrate, in DMSO
- Enolase Substrate Mix
- Enolase Converter
- Enolase Developer
- Enolase Positive Control
- Hydrogen Peroxide Standard, 0.88 M
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
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