Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

View All Documentation

E2886

Sigma-Aldrich

ExtrAvidin®−Peroxidase

buffered aqueous solution

Synonyme(s) :

ExtrAvidin HRP

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Numéro MDL:
MFCD00163600
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Conjugué

peroxidase conjugate

Niveau de qualité

200

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Spécificité

The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.

Application

ExtrAvidin®-Peroxidase is suitable for the following applications:
  • Dot blot at a minimum dilution of 1:4,000
  • Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
  • Direct ELISA at a minimum dilution of 1:2,000
It has also been used for:
  • Immunoprecipitation and Immunobloting
  • Ra2I solid-phase binding assays
  • Southwestern blotting
  • Western blotting
  • Immunocytochemistry

Actions biochimiques/physiologiques

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Notes préparatoires

Affinity purified protein

Stockage et stabilité

Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Informations légales

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Dimer-tetramer transition between solution and crystalline states of streptavidin and avidin mutants.
Pazy Y, et al.
Journal of Bacteriology, 185(14), 4050-4056 (2003)
G Grouard et al.
The Journal of experimental medicine, 185(6), 1101-1111 (1997-03-17)
A subset of CD4+CD11c-CD3- blood cells was recently shown to develop into dendritic cells when cultured with monocyte conditioned medium. Here, we demonstrate that CD4+ CD11c-CD3- cells, isolated from tonsils, correspond to the so-called plasmacytoid T cells, an obscure cell
S Correia et al.
Molecular human reproduction, 19(9), 581-589 (2013-04-26)
Sperm undergo maturation acquiring progressive motility and the ability to fertilize oocytes through exposure to the components of the epididymal fluid (EF). Although the establishment of a calcium (Ca(2+)) gradient along the epididymis has been described, its direct effects on
Peter Hauff et al.
Radiology, 231(3), 667-673 (2004-05-01)
To evaluate the feasibility of using intravenously administered L-selectin ligand-specific polymer-stabilized air-filled microparticles (MPs) for active targeting of peripheral lymph nodes under normal conditions in animal models. L-selectin ligand-specific MPs and two control substances (immunoglobulin M-isotype MPs and native MPs)
M C Dieu-Nosjean et al.
The Journal of experimental medicine, 192(5), 705-718 (2000-09-07)
Dendritic cells (DCs) form a network comprising different populations that initiate and differentially regulate immune responses. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium, and we present here observations suggesting that macrophage inflammatory protein (MIP)-3alpha plays a
Afficher tous les articles scientifiques apparentés

Protocoles

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique