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MAK134

Sigma-Aldrich

Ketone Body Assay

sufficient for 200 tests (UV)

Synonyme(s) :

Ketone Quantification Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 200 tests (UV)

Caractéristiques

This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; neonatal diseases; pediatric diseases; cancer

Température de stockage

−20°C

Catégories apparentées

Description générale

Ketone bodies, 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc), are produced in the liver primarily from oxidation of fatty acids, and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypoglycemia, growth hormone deficiency, alcohol or salicylate intoxication, and inborn errors of metabolism.

Application

Ketone Body Assay has been used to measure the release of ketone bodies in the human liver cancer cell line HepG2 culture medium.

Caractéristiques et avantages

Compatible with high-throughput handling systems

Adéquation

Suitable for the detection of ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) in serum, plasma, urine, and other biological samples.

Principe

In this kit, AcAc and BOH levels are determined using an enzymatic assay based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance measured at 340 nm is directly related to either the AcAc and BOH concentrations.

Code de la classe de stockage

10 - Combustible liquids

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Certificats d'analyse (COA)

Lot/Batch Number
134CF01A08
134CF01A08
134CE06A28
134CE06A28
134CD11A21

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Consulter la Bibliothèque de documents

Hyperketonemia and ketosis increase the risk of complications in type 1 diabetes.
Kanikarla M, et al.
Free Radical Biology & Medicine, 95, 268-277 (2016)
Zachary M Augur et al.
Frontiers in nutrition, 5, 91-91 (2018-10-24)
Introduction: Temozolomide (TMZ) is part of the standard of care for treating glioblastoma multiforme (GBM), an aggressive primary brain tumor. New approaches are needed to enhance therapeutic efficacy and reduce toxicity. GBM tumor cells are dependent on glucose and glutamine
Role of β-hydroxybutyrate, its polymer poly-β-hydroxybutyrate and inorganic polyphosphate in mammalian health and disease.
Dedkova E N and Lothar A B
Frontiers in Physiology, 5, 260-260 (2014)
Kim J Shimy et al.
Journal of the Endocrine Society, 4(7), bvaa062-bvaa062 (2020-07-16)
According to the carbohydrate-insulin model of obesity, an elevated insulin-to-glucagon ratio in response to a high-carbohydrate diet directs metabolic fuels toward storage, resulting in lower circulating energy. To determine differences in total circulating energy post-meal related to dietary carbohydrate. Ancillary
Anila K Madiraju et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(24), E3423-E3430 (2016-06-02)
A key sensor of cellular energy status, AMP-activated protein kinase (AMPK), interacts allosterically with AMP to maintain an active state. When active, AMPK triggers a metabolic switch, decreasing the activity of anabolic pathways and enhancing catabolic processes such as lipid
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