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MAK053

Sigma-Aldrich

Alcohol Dehydrogenase Activity Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

gastrointestinal diseases

Température de stockage

−20°C

Informations sur le gène

human ... ADH1A(124) , ADH1B(125) , ADH1C(126) , ADH4(127) , ADH5(128) , ADH6(130) , ADH7(131)
mouse ... ADH1C(11522) , ADH4(26876) , ADH5(11532) , ADH6A(69117) , ADH7(11529)
rat ... ADH1C(24172) , ADH4(29646) , ADH5(100145871) , ADH6(310903) , ADH6A(295498) , ADH7(171178)

Description générale

The new Alcohol Dehydrogenase (ADH) Assay Kit, MAK498, is now available! Alcohol dehydrogenases (ADH) are a family of enzymes that catalyzes the conversion of alcohols to aldehydes, with the concomitant reduction of NAD+ to NADH. In humans, there are nine isozymes of ADH, with the majority of ADH activity occurring in the liver. ADH family members are the primary enzymes involved in alcohol detoxification. Genetic variations in ADH enzymes result in differences in ADH activity and tolerances for alcohol, and may regulate susceptibility to alcoholism.

Application

Alcohol Dehydrogenase Activity Assay Kit has been used to determine the activity of alcohol dehydrogenase.

Adéquation

Suitable for the detection of alcohol dehydrogenase in a variety of samples including tissue, cells and serum.

Principe

The Alcohol Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring ADH activity in a variety of samples. ADH activity is determined using ethanol as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of ADH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at 37 °C.

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Réf. du produit
Description
Tarif

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Mentions de danger

H225,H319,H412

Classification des risques

Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

53.6 °F - closed cup

Point d'éclair (°C)

12.0 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Kobi Simpson-Lavy et al.
Scientific reports, 9(1), 6491-6491 (2019-04-26)
Cells adapt their gene expression and their metabolism in response to a changing environment. Glucose represses expression of genes involved in the catabolism of other carbon sources in a process known as (carbon) catabolite repression. However, the relationships between "poor"
Rachel E N Reyes et al.
Brain research, 1747, 147067-147067 (2020-08-23)
Research focusing on the gut-brain axis is growing, but the interplay of ethanol (alcohol molecule), the gut microbiome, the brain and behavior is poorly understood. In the current study, we remodeled the gut microbiota by providing adult male C57BL/6J mice
Alcohol increases liver progenitor populations and induces disease phenotypes in human IPSC-derived mature stage hepatic cells
Tian L, et al.
International Journal of Biological Sciences, 12(9), 1052-1052 (2016)
Christopher M Ranger et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(17), 4447-4452 (2018-04-11)
Animal-microbe mutualisms are typically maintained by vertical symbiont transmission or partner choice. A third mechanism, screening of high-quality symbionts, has been predicted in theory, but empirical examples are rare. Here we demonstrate that ambrosia beetles rely on ethanol within host
Sang-Hyuk Hong et al.
Phytotherapy research : PTR, 31(5), 783-791 (2017-03-17)
Although Pinus koraiensis leaf (PKL) was reported for its anti-diabetes, anti-obesity and anticancer effects as a folk remedy, the inhibitory effect of PKL on alcoholic fatty liver has never been elucidated yet. This study investigated the molecular mechanisms of PKL
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Protocoles

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

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