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M8662

Sigma-Aldrich

Huile minérale

PCR Reagent

Synonyme(s) :

Mineral oil for PCR

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About This Item

Numéro CAS:
8042-47-5
Numéro CE :
232-455-8
Numéro MDL:
MFCD00131611
Code UNSPSC :
12181504
Nomenclature NACRES :
NA.52

Qualité

PCR Reagent

Niveau de qualité

100

Forme

liquid

Conditionnement

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

Technique(s)

PCR: suitable

Couleur

colorless

Indice de réfraction

n20/D 1.467 (lit.)

Densité

0.84 g/mL at 25 °C (lit.)

Activité étrangère

DNase, RNase, protease, none detected

Température de stockage

room temp

Clé InChI

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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Catégories apparentées

Description générale

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

Application

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform

Caractéristiques et avantages

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Ivan K Dimov et al.
Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Hartmut Kaiser et al.
Plant physiology, 143(2), 1068-1077 (2006-12-13)
The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
Hsin-I Jen et al.
eLife, 8 (2019-04-30)
The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
Decagram scale production of deuterated mineral oil and polydecene as solvents for polymer studies in neutron scattering
Klenner MA, et al.
Open Journal of Polymer Chemistry, 11(31), 4986-4994 (2020)
Neus Bota-Rabassedas et al.
Cell reports, 35(3), 109009-109009 (2021-04-22)
Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
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Protocoles

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Afficher tout

Contenu apparenté

KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

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