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M5310

Sigma-Aldrich

Huile minérale

light oil, suitable for mouse embryo cell culture, BioXtra

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About This Item

Numéro CAS:
8042-47-5
Numéro CE :
232-455-8
Numéro MDL:
MFCD00131611
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Stérilité

sterile-filtered

Niveau de qualité

300

Gamme de produits

BioXtra

Forme

light oil

Conditions de stockage

protect from light

Technique(s)

cell culture | embryo: suitable

Indice de réfraction

n20/D 1.467 (lit.)

Densité

0.82-0.88 g/mL at 25 °C

Format

neat

Clé InChI

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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Description générale

Mineral oil or liquid petroleum is used for conservation of various types of cutltures. The advantages of using mineral oil are that the growth rate is decreased and the evaporation of water from the medium is almost completely eliminated. Thus, sub-culturing is reduced leading to increase in the viability of the culture. The first time mineral oil was used was in 1914, and it was used to overlay bacterial culture.

Application

Mineral oil has been commonly used and recommended for overlaying mouse embryo cultures to prevent drying during the procedure.
Mineral oil is used for the following applications:
  • Used for cell viability studies in preparing the culture media or media supplements
  • Used tp protect array from evaporation by adding mineral oil around the array
  • Used to prepare the needle for microinjection (microinjection needle pulling, sharpening, and loading)
  • Used in PCR setup

Caractéristiques et avantages

Ready-to-use sterile filtered product conveniently packaged in PET bottles.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

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Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Slobodan Beronja et al.
Methods in molecular biology (Clifton, N.J.), 961, 351-361 (2013-01-18)
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in
Bei Zhao et al.
Scientific reports, 7(1), 11319-11319 (2017-09-14)
A microfluidic chip with a controllable and integrated piezoelectric pump was proposed and demonstrated, where the pump was designed as a micro-actuator based on polyvinylidene fluoride (PVDF) organic piezoelectric film. In this case, the pump should integrate with the microfluidics
Hanyoup Kim et al.
Optics express, 17(1), 218-227 (2009-01-09)
We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature
Xiyan Wang et al.
Journal of clinical laboratory analysis, 34(11), e23498-e23498 (2021-01-20)
Efficiency of preantral follicle culture in vitro is low and is dependent on species, development stage, and follicle-stimulating hormone (FSH) concentration. Here, we optimized the preantral follicle in vitro culture system in mice. The primary follicles (PM follicles, 80-100 μm
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