M5909
IgM Isotype Control from murine myeloma
clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin
Synonyme(s) :
Mouse IgM Isotype Control from myeloma
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Clone
MOPC 104E, monoclonal
Forme
buffered aqueous solution
Concentration
200 μg/mL
Technique(s)
flow cytometry: suitable
Isotype
IgMλ
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Description générale
IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory . This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice . IgM isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgM. The product does not react with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3.
Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
The purified IgM Isotype Control clone MOPC 104E preparation is non-reactive with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. When evaluated in flow cytometry, the IgM Isotype Control did not stain human peripheral blood lymphocytes (PBLs). A FITC Goat anti-Mouse IgM (μ-chain specific), Affinity Isolated Antibody (Product No. F 9259) along with 1 μg of the product was incubated with human PBLs and then evaluated by flow cytometry.
Application
IgM Isotype Control clone MOPC 104E is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. Equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry.
IgM Isotype Control from murine myeloma has been used:
- in chromatin immunoprecipitation (ChIP) assay
- LABScreen assays
- immunofluorescent staining
IgM isotype control antibodies are suitable for use in immunohistochemistry .
Principe
The specificity of staining by monoclonal antibodies to mouse CD antigens should be verified by establishing the non-specific reagent binding to the target cell population. It is recommended that a concentration and isotype matched mouse myeloma immunoglobulin be included as a negative control for each monoclonal antibody reagent used in the immunoassay procedure.
Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. DUAL-TAG controls contain 100 μg of each conjugate.
Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. DUAL-TAG controls contain 100 μg of each conjugate.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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