LYSC9000

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

• Synonyme(s) : Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
• Numéro de classification (Commission des enzymes): 3.4.21.50 (BRENDA, IUBMB)
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Achromobacter lyticus
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli (206-473aa)
• Qualité: Proteomics Grade
• Forme: ready-to-use solution
• Concentration: 1 mg/mL
• pH optimal: 8.5
• Plage de pH: 9.0-9.5(catalytic)
• Numéro d'accès UniProt: P15636
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Spécificité

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Application

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Conditionnement

1mg/ml in Plastic

Notes préparatoires

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

Stockage et stabilité

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

Autres remarques

For R&D only.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable