L7525

L-Lactic Dehydrogenase from porcine heart

ammonium sulfate suspension, ≥200 units/mg protein

• Synonyme(s) : (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD, Lactate
• Numéro CAS: 9001-60-9
• Numéro de classification (Commission des enzymes): 1.1.1.27 (BRENDA, IUBMB)
• Numéro CE : 232-617-8
• Numéro MDL: MFCD00131466
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Porcine heart
• Niveau de qualité: 200
• Forme: ammonium sulfate suspension
• Activité spécifique: ≥200 units/mg protein
• Numéro d'accès UniProt: P00339
• Activité étrangère: glutamic-oxalacetic transaminase ≤0.1%; malic dehydrogenase, glutamic-pyruvic transaminase and pyruvate kinase ≤0.06%
• Température de stockage: 2-8°C
• Informations sur le gène: pig ... LDHA(407245)

Description

Description générale

Research area: Cell Signaling

Lactic Dehydrogenase (LDH) is a cytoplasmic enzyme encoded by LDHA, LDHB, LDHC, and LDHD genes, and categorized as an oxidoreductase. It is widely distributed throughout the body, particularly in muscle, liver, and kidney. LDH demonstrates five isomeric forms that form tetramers with two types of subunits: muscle (M) and heart (H).

Application

L-Lactic Dehydrogenase from porcine heart has been used:

• as a component of the reaction buffer to measure the GTPase activity of EngA bound to the bacterial 50S subunit to study its structure and function.
• as a component of the enzyme solutions to test the sensitivity of multiphoton NAD(P)H fluorescence lifetime imaging (FLIM) in key enzymatic steps controlling the path of carbon from glucose uptake to electron transport chain (ETC) activity.

Actions biochimiques/physiologiques

Lactate dehydrogenase (LDH) enzyme is responsible for the conversion of pyruvate to lactate, during glycolysis under hypoxic conditions, while also converting NADH to NAD⁺. Moreover it acts as a crucial regulator of gluconeogenesis and DNA metabolism. Increased serum LDH levels are detected in conditions such as cancer, HIV infection, muscular dystrophy, megaloblastic anemia, extreme hypothermia, hepatitis, meningitis, hypoxia, etc.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Définition de l'unité

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Forme physique

Suspension in ammonium sulfate and 0.1 M potassium phosphate, pH 7.0

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)