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Key Documents

L3023

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4

Detoxified

Synonyme(s) :

LPS

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About This Item

Numéro CE :
297-473-0
Numéro MDL:
MFCD00164401
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25

Source biologique

Escherichia coli (O111:B4)

Niveau de qualité

200

Forme

lyophilized powder

Couleur

white to beige

Solubilité

water: soluble

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

This product is extracted from E. coli serotype O111:B4 and purified by chromatography. The source strain is from a private collection. The lipid A moiety has been partially delipidated by alkaline hydrolysis. Removal of the fatty acid portions of lipid A results in a detoxified LPS with an endotoxin level about 10,000 times lower than that of the parent LPS.

Application

Les lipopolysaccharides (LPS) sont des éléments constitutifs caractéristiques de la paroi cellulaire des bactéries à Gram négatif. Les LPS et leur fraction lipidique (lipide A) stimulent les cellules du système immunitaire inné par l'intermédiaire du récepteur TLR4 (Toll-Like Receptor 4), qui appartient à la famille des récepteurs de type Toll et qui reconnaît la signature moléculaire des principaux agents pathogènes (PAMP).

Actions biochimiques/physiologiques

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Notes préparatoires

Chromatographically purified; delipidized by alkaline hydrolysis
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

L4391

Lipopolysaccharides from Escherichia coli O111:B4, γ-irradiated, BioXtra, suitable for cell culture

L3012

Lipopolysaccharides from Escherichia coli O111:B4, purified by gel-filtration chromatography

L4130

Lipopolysaccharides from Escherichia coli O111:B4, purified by trichloroacetic acid extraction

L3024

Lipopolysaccharides from Escherichia coli O111:B4, purified by ion-exchange chromatography, TLR ligand tested

L2630

Lipopolysaccharides from Escherichia coli O111:B4, purified by phenol extraction

L3023

Lipopolysaccharides from Escherichia coli O111:B4, Detoxified

F3665

Lipopolysaccharides from Escherichia coli O111:B4, FITC conjugate

L5293

Lipopolysaccharides from Escherichia coli O111:B4, Ready Made solution, 1 mg/mL

Pictogrammes

Skull and crossbones
GHS06

Mention d'avertissement

Danger

Mentions de danger

H300

Classification des risques

Acute Tox. 2 Oral

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Hua Qiu et al.
Drug design, development and therapy, 13, 589-600 (2019-02-28)
Jie-Du-Hua-Yu (JDHY) granule is a combination of six traditional Chinese medicines with known therapeutic effect in treating acute liver failure (ALF). The aim of this study was to investigate the amelioration efficacy of JDHY in lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced ALF in rat
Carol L Ladner-Keay et al.
Prion, 10(6), 466-483 (2016-12-03)
Conversion of native cellular prion protein (PrPc) from an α-helical structure to a toxic and infectious β-sheet structure (PrPSc) is a critical step in the development of prion disease. There are some indications that the formation of PrPSc is preceded
Gabrielle R Andrade et al.
Human vaccines & immunotherapeutics, 10(10), 2864-2874 (2014-12-09)
E. coli O111 strains are responsible for outbreaks of blood diarrhea and hemolytic uremic syndrome throughout the world. Because of their phenotypic variability, the development of a vaccine against these strains which targets an antigen that is common to all
Fernando F Anhê et al.
Cell reports, 36(11), 109691-109691 (2021-09-16)
Lipopolysaccharides (LPSs) can promote metabolic endotoxemia, which is considered inflammatory and metabolically detrimental based on Toll-like receptor (TLR)4 agonists, such as Escherichia coli-derived LPS. LPSs from certain bacteria antagonize TLR4 yet contribute to endotoxemia measured by endotoxin units (EUs). We
Rhonda H Wilson et al.
American journal of respiratory and critical care medicine, 180(8), 720-730 (2009-08-08)
In humans, immune responses to inhaled aeroallergens develop in the lung and draining lymph nodes. Many animal models of asthma bypass this route and instead use intraperitoneal injections of allergen using aluminum hydroxide as an adjuvant. We investigated whether allergic
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Contenu apparenté

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

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