L9641

Lipopolysaccharides (rough strains) from Escherichia coli EH100 (Ra mutant)

• Synonyme(s) : LPS
• Numéro CE : 297-473-0
• Numéro MDL: MFCD00164401
• Code UNSPSC : 12352201
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: Escherichia coli (EH100 Ra mutant)
• Niveau de qualité: 200
• Forme: lyophilized powder
• Impuretés: <3% Protein (Lowry)
• Couleur: white to yellow
• Solubilité: water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

This product is phenol:chloroform:petroleum ether extracted from E. coli EH 100 (Ra mutant). The source strain is from a private collection.

Actions biochimiques/physiologiques

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Notes préparatoires

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informations sur la sécurité

• Pictogrammes: GHS06
• Mention d'avertissement: Danger
• Mentions de danger: H300
• Conseils de prudence: P264 - P270 - P301 + P310 - P405 - P501
• Classification des risques: Acute Tox. 2 Oral
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)