I5271

IL-1β mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : IL-1β, Lymphocyte activating factor
• Numéro MDL: MFCD00164815
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• product name: Interleukin-1β from mouse, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Pureté: ≥97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: ≤0.010 ng/mL ED₅₀
• Qualité: endotoxin tested
• Poids mol.: 17.5 kDa (containing 153 amino acid residues)
• Conditionnement: pkg of 5X5 μg; pkg of 5 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤0.1ng/mg
• Numéro d'accès UniProt: P10749
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Il1b(16176)

Description

Description générale

Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa.

Application

Interleukin-1β from mouse has been used:

• as a proinflammatory agent, to induce inflammatory signaling cascades mediated cellular damage
• to study its effect on fetuin A-induced inflammatory response in podocyte
• to check its effect on the expression of mitogen-activated protein kinase 4 using 3T3-L1 adipocytes

Actions biochimiques/physiologiques

Interleukin-1β (IL-1β) is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.

Forme physique

Lyophilized from 0.2 μm filtered solution in 10mM Sodium Citrate, pH 4.0

Remarque sur l'analyse

The biological activity is measured by the dose-dependent stimulation of murine D10G4.1 cells.

Autres remarques

The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable