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I2771

Sigma-Aldrich

ITS+3 Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonyme(s) :

ITS Supplement

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About This Item

Code UNSPSC :
12352205
Nomenclature NACRES :
NA.75

Stérilité

sterile-filtered

Niveau de qualité

300

Gamme de produits

BioReagent

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

ITS+3 liquid media supplement is a mixture of insulin, human transferrin (partially iron-saturated), sodium selenite, linoleic acid, and bovine serum albumin (BSA). It is suitable for serum-free cell culture applications.

Application

ITS+3 Liquid Media Supplement (100×) has been used as a media supplement:
  • to support the growth of human adipose stem cells (ASC)
  • to culture porcine intervertebral disc (IVD) cells and tissues
  • to culture cartilage endplate (CEP) cells

Actions biochimiques/physiologiques

  • Insulin: a polypeptide hormone that promotes glucose and amino acid uptake and may produce a mitogenic effect.
  • Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
  • Selenium: an essential trace element present in serum.

Composants

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 470 μg/ml linoleic acid, 470 μg/ml oleic acid and 50 mg/ml bovine serum albumin at the 100x concentration. Contains 2 moles each of linoleic acid and oleic acid per mole of albumin.

Notes préparatoires

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Adam Cornish et al.
BMC genomics, 21(Suppl 11), 830-830 (2020-12-30)
Single-cell sequencing enables us to better understand genetic diseases, such as cancer or autoimmune disorders, which are often affected by changes in rare cells. Currently, no existing software is aimed at identifying single nucleotide variations or micro (1-50 bp) insertions and
Xue Liu et al.
iScience, 24(6), 102551-102551 (2021-06-22)
Pulmonary mesenchymal cells are critical players in both the mouse and human during lung development and disease states. They are increasingly recognized as highly heterogeneous, but there is no consensus on subpopulations or discriminative markers for each subtype. We completed
Di Wu et al.
International journal of stem cells, 14(1), 119-126 (2020-12-31)
The research on human hepatobiliary development and disorders has been constrained by minimal access to human fetal tissue, and low accuracy of animal models. To overcome this problem, we have established a system for the differentiation of human pluripotent stem
Katherine Lakstins et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 39(6), 1204-1216 (2020-04-15)
Cartilage endplate degeneration/calcification has been linked to the onset and progression of intervertebral disc degeneration and there is a critical need to understand mechanisms, such as hypertrophic differentiation, of cartilage endplate degeneration/calcification to inform treatment strategies for discogenic back pain.
Hien Lau et al.
Islets, 12(3), 41-58 (2020-05-28)
The use of pancreata from pre-weaned piglets has the potential to serve as an unlimited alternative source of islets for clinical xenotransplantation. As pre-weaned porcine islets (PPIs) are immature and require prolonged culture, we developed an islet maturation media (IMM)
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