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Key Documents

L0288

Sigma-Aldrich

Lipid Mixture 1, Chemically Defined

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonyme(s) :

ACF lipid mixture

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About This Item

Code UNSPSC :
12352211
Nomenclature NACRES :
NA.75

Source biologique

non-animal source

Niveau de qualité

Stérilité

sterile-filtered

Gamme de produits

BioReagent

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤10 EU/mL endotoxin

Température de stockage

2-8°C

Catégories apparentées

Description générale

Lipid mixture 1, Chemically defined is recommended for use in cell culture at 1 to 10 mL per liter of medium.

Application

Lipid Mixture 1, Chemically Defined has been used:

  • as a component of bovine serum albumin (BSA) containing Bracket-Oliphant (BO) medium for the resuspension of sperm pellet
  • as a supplement in spheroid media for culturing primary human subcutaneous stromal vascular fraction cells
  • to prepare a cell steatosis model for culturing of mouse and human hepatocytes

Autres remarques

Contains non-animal derived fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic), 0.22 mg/ml cholesterol from New Zealand sheep′s wool, 2.2 mg/ml Tween-80, 70 μg/ml tocopherol acetate and 100 mg/ml Pluronic F-68 solubilized in cell culture water.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Juan Zhong et al.
International immunopharmacology, 59, 197-208 (2018-04-16)
The anti-inflammatory, immunomodulatory, and anticancer effects of micheliolide (MCL) isolated from Michelia champaca were previously reported, but its role and underlying mechanisms in relieving liver steatosis remain unclear. Herein, we investigated the effects of MCL on hepatic steatosis using a
Anna Ioannidou et al.
The Journal of physiology (2021-08-14)
Adipocyte enlargement is a key feature of obesity and associated with insulin resistance and metabolic disease The cause and consequences of adipocyte enlargement have remained hard to study in vitro due to a lack of human cell models with representative
Marc P Hübner et al.
PLoS neglected tropical diseases, 14(7), e0008427-e0008427 (2020-07-07)
A major impediment to eliminate lymphatic filariasis and onchocerciasis is the lack of effective short-course macrofilaricidal drugs or regimens that are proven to be safe for both infections. In this study we tested oxfendazole, an anthelmintic shown to be well
Md Mazharul Islam et al.
Reproductive medicine and biology, 20(3), 289-298 (2021-07-16)
The present study was undertaken to clarify whether bovine sperm could take up fatty acids (FAs) and produce ATP to maintain linear motility. Frozen bovine semen was thawed in media containing either lipid mixture (LM) or FAs, and sperm motility
Semir Beyaz et al.
Nature, 531(7592), 53-58 (2016-03-05)
Little is known about how pro-obesity diets regulate tissue stem and progenitor cell function. Here we show that high-fat diet (HFD)-induced obesity augments the numbers and function of Lgr5(+) intestinal stem cells of the mammalian intestine. Mechanistically, a HFD induces

Articles

Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

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