GE28-9909-46

Superdex^® 200 Increase 3.2/300

column L × I.D. 30 cm × 3.2 mm, 8.6 μm particle size

• Synonyme(s) : Superdex 200 Increase
• Code UNSPSC : 41115700
• Nomenclature NACRES : SB.52

Propriétés

• Matériaux: glass column; titanium frit
• Gamme de produits: Superdex^®
• Poids mol.: 10000-600000 Da (globular proteins)
• Conditionnement: 1 ea of
• Paramètres: <0.15 ml/min flow rate; 3.0 Mpa (652 psi); 4-50 mL sample volume
• Volume du lit: ~2.4 mL
• Colonne, L × D.I.: 30 cm × 3.2 mm
• Matrice: agarose-dextran composite; polymeric
• Taille des particules: 8.6 μm
• cleaning: 1-14
• working range: 3-12
• Technique de séparation: size exclusion (SEC)

Description

Description générale

Superdex™ 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.

Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.

Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.

Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.

This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Caractéristiques et avantages

• Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
• Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
• High flow rates: rigid beads give excellent pressure/flow properties.

Adéquation

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride

for both preparative and analytical purposes

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

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Informations légales

Superdex is a registered trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids