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GE28-9523-60

GST GraviTrap™

Cytiva 28-9523-60

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About This Item

Code UNSPSC :
41106501
Nomenclature NACRES :
NA.56

ligand

glutathione

Conditionnement

pkg of 10 ea

Fabricant/nom de marque

Cytiva 28-9523-60

Volume du lit

2 mL

D.I. de la colonne

15 mm

Matrice

4% agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning

4-13

working range

4-13

Capacité

≥20 mg binding capacity(recombinant glutathione S-transferase (rGST))
 >25 mg binding capacity (horse liver GST/ml medium)

Description générale

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion protein.

Application

GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap™ columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

Caractéristiques et avantages

  • Convenient, prepacked gravity-fLow columns.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • Yields up to 20 mg of pure Proteins per column.
  • Yields up to 20 mg of pure Proteins per column

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Afficher tout

Protocoles

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

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