GE17-5282-02
GSTrap™ High Performance
Cytiva 17-5282-02, pack of 5 × 5 mL
About This Item
Produits recommandés
ligand
glutathione
Conditionnement
pack of 5 × 5 mL
Fabricant/nom de marque
Cytiva 17-5282-02
Paramètres
<20 mL/min flow rate
42 psi (H2O at room temperature.)
Taille du lit
16 mm × 25 mm
Volume du lit
5 mL
D.I. de la colonne
16 mm
Matrice
highly cross-linked 6% agarose
Taille moy. des particules
34 μm
cleaning
3-12
working range
3-12
Capacité
>7 mg/mL binding capacity (medium)
Catégories apparentées
Description générale
GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose™ High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.
GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
Caractéristiques et avantages
- High resolution purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Fast, one-step purification.
- Easy to use with syringe, pump or a chromatography system.
Stockage et stabilité
Remarque sur l'analyse
Autres remarques
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Protocoles
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.
GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.
GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.
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