GE28-9187-79

MBPTrap^™ HP

Cytiva 28-9187-79, pack of 1 × 5 mL

• Code UNSPSC : 23151816
• Nomenclature NACRES : NA.56

Propriétés

• ligand: dextrin
• Conditionnement: pack of 1 × 5 mL
• Fabricant/nom de marque: Cytiva 28-9187-79
• Conditions de stockage: (20% Ehtanol)
• Paramètres: <20 mL/min flow rate; 42 psi
• Taille du lit: 16 mm × 25 mm
• Volume du lit: 5 mL
• D.I. de la colonne: 16 mm
• Matrice: highly cross-linked 6% agarose
• Taille moy. des particules: 34 μm
• cleaning: 2-13
• working range: >7
• Capacité: ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
• Température de stockage: 2-8°C

Description

Description générale

MBPTrap™ HP columns prepacked with Dextrin Sepharose™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.

Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Caractéristiques et avantages

• HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium.
• Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
• Physiological conditions and mild elution preserve the activity of the target protein.
• Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

MBPTrap is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids