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Key Documents

GE28-9031-35

Protein G High Performance MultiTrap™

Cytiva 28-9031-35

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About This Item

Code UNSPSC :
41105323
Nomenclature NACRES :
NA.25

ligand

recombinant protein G lacking albumin-binding region

Matériaux

polypropylene and polyethylene

Caractéristiques

wetted part: no

Conditionnement

pkg of 4 ea

Fabricant/nom de marque

Cytiva 28-9031-35

Conditions de stockage

(20% Ehtanol)
 (20% Ethanol)

Paramètres

30 °C max. temp.
4 °C min. temp.

Taille

127.8 mm × 85.5 mm × 30.6 mm

Matrice

highly cross-linked 6% agarose

Taille moy. des particules

34 μm

cleaning

2-9

working range

3-9

Capacité

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)

Température de stockage

2-8°C

Description générale

Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species.

Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).

A protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min is also provided. No pretreatment of the sample is required.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caractéristiques et avantages

  • Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
  • Flexible format, protocol for classical elution as well as cross-linking method.
  • Formatted for elution for electrophoresis or LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.
  • Prepacked with Protein G Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
  • MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiTrap is a registered trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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Articles

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique