GE28-9031-32

Protein A High Performance Spintrap^™

Cytiva 28-9031-32, pack of 16 columns

• Synonyme(s) : High Performance Spintrap, Protein A Spintrap
• Code UNSPSC : 41106500
• Nomenclature NACRES : NA.25

Propriétés

• ligand: native protein A
• Caractéristiques: wetted part: no
• Conditionnement: pack of 16 columns
• Fabricant/nom de marque: Cytiva 28-9031-32
• Conditions de stockage: (20% Ethanol)
• Matrice: highly cross-linked 6% agarose
• Taille moy. des particules: 34 μm
• cleaning: 2-10
• working range: 3-9
• Capacité: >1 mg binding capacity(human IgG well)
• Température de stockage: 2-8°C

Description

Description générale

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

protein A HP SpinTrap^™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose^™ HP.

Application

Protein A Sepharose^™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Caractéristiques et avantages

• For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
• Classic and cross-link protocols provide flexibility.
• Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
• Fast binding kinetics and high capacity provide high yield.
• Prepacked with Protein A Sepharose^™ High Performance for coupling of antibodies of IgG subclasses.
• Easy scale-up with HiTrap^® Protein A HP prepacked columns.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids