G6642
Anti-Glutamate antibody produced in rabbit
whole antiserum
Synonyme(s) :
Anti-Glutamate Ab, Glutamate Detection Antibody, Rabbit Anti-Glutamate
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
whole antiserum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Contient
15 mM sodium azide
Espèces réactives
wide range
Technique(s)
dot blot: 1:15,000
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Catégories apparentées
Description générale
The amino acids L-glutamate (Glu) and L-aspartate (Asp) are considered the major excitatory neurotransmitters in the central nervous system (CNS) and represent the most abundant mammalian neurotransmitter class. Both L-glutamate and L-aspartate are present in the brain at high concentrations and are distributed in most excitatory pathways in the CNS. Glu- and Asp-immunoreactivities are localized in high concentrations in synaptic terminals. In nerve terminals, L-glutamate is formed by deamidation of its major precursor, L-glutamine, by the enzyme glutaminase.
The actions of the excitatory amino acids on neurons are mediated by different receptor subtypes. These receptors are coupled to integral ion channels or to a second messenger system which utilizes inositol triphosphate (IP3). L-glutamate and L-aspartate may play an important role in the pathogenesis of certain neurological disorders such as Huntington′s disease, Alzheimer′s disease, epilepsy and brain ischemia. The excitoxic and neurotoxic effects of L-glutamate, leading to extensive neuronal damage, appear to be mediated by the N-methyl-D-aspartate (NMDA) receptor subtype.
The actions of the excitatory amino acids on neurons are mediated by different receptor subtypes. These receptors are coupled to integral ion channels or to a second messenger system which utilizes inositol triphosphate (IP3). L-glutamate and L-aspartate may play an important role in the pathogenesis of certain neurological disorders such as Huntington′s disease, Alzheimer′s disease, epilepsy and brain ischemia. The excitoxic and neurotoxic effects of L-glutamate, leading to extensive neuronal damage, appear to be mediated by the N-methyl-D-aspartate (NMDA) receptor subtype.
Spécificité
The antiserum is evaluated for activity and specificity by dot-blot immunoassay. The antiserum recognizes L-glutamic acid immobilized on an affinity membrane. No cross-reaction is observed with L-aspartic acid, L-glutamine, L-asparagine, and L-alanine. Weak cross-reactivity is observed with Gly-Asp, GABA, β-alanine, glycine and 5-aminovaleric acid (amino acid concentration 5-10 mM). Weak to moderate reaction is observed with L-glutamic acid-containing dipeptides Asp-Glu and Gly-Glu. The antibody may be used to localize glutamate in cell bodies, axons, and terminals of glutamatergic neurons in the CNS.
Immunogène
L-glutamic acid (Glu).
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Rabbit polyclonal antiserum to L-glutamate may be used in immunohistochemical techniques on formalin- or glutaraldehyde-fixed, vibratome or frozen sections of human or animal tissues. Immunohistochemical methods provide increased anatomical resolution over conventional biochemical methods.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Juan Guo et al.
EBioMedicine, 65, 103272-103272 (2021-03-11)
Despite the fundamental clinical significance of general anaesthesia, the cortical mechanism underlying anaesthetic-induced loss of consciousness (aLOC) remains elusive. Here, we measured the dynamics of two major cortical neurotransmitters, gamma-aminobutyric acid (GABA) and glutamate, through in vivo two-photon imaging and
Marta Słoniecka et al.
PloS one, 10(7), e0134157-e0134157 (2015-07-28)
Keratocytes, the quiescent cells of the corneal stroma, play a crucial role in corneal wound healing. Neuropeptides and neurotransmitters are usually associated with neuronal signaling, but have recently been shown to be produced also by non-neuronal cells and to be
Èlia Benito-Gutiérrez et al.
BMC biology, 19(1), 110-110 (2021-05-23)
The evolutionary origin of the telencephalon, the most anterior part of the vertebrate brain, remains obscure. Since no obvious counterpart to the telencephalon has yet been identified in invertebrate chordates, it is difficult to trace telencephalic origins. One way to
Wenjun Zhou et al.
Nature neuroscience, 22(2), 205-217 (2019-01-22)
Nuclear receptor corepressor 1 (NCOR1) and NCOR2 (also known as SMRT) regulate gene expression by activating histone deacetylase 3 through their deacetylase activation domain (DAD). We show that mice with DAD knock-in mutations have memory deficits, reduced anxiety levels, and
Jonathan E Elliott et al.
Sleep, 41(3) (2018-01-10)
In previous work, dietary branched-chain amino acid (BCAA) supplementation, precursors to de novo glutamate and γ-aminobutyric acid (GABA) synthesis, restored impaired sleep-wake regulation and orexin neuronal activity following traumatic brain injury (TBI) in mice. TBI was speculated to reduce orexin
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