Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

G4546

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

GFAP Antibody for Flow Cytometry - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, Gfap Antibody Flow Cytometry, Anti-FLJ45472, Anti-GFAP, Anti-Intermediate filament protein

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD00145904
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~50 kDa

Espèces réactives

human, rat, mouse

Concentration

~1 mg/mL

Technique(s)

flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000

Numéro d'accès UniProt

P14136

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... GFAP(2670)
mouse ... Gfap(14580)
rat ... Gfap(24387)

Description générale

Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological, and biochemical criteria. Intermediate filaments differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. Glial Fibrillary Acidic Protein (GFAP) (molecular weight of 50 kDa) is found in astrocytes and ependymal cells of the central nervous system. It is found in various other cell types including stellate cells, chondrocytes, osteocytes, keratinocytes, Leydig cells and fibroblasts. GFAP is involved in mitosis, and cell:cell interactions and communications. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.

Spécificité

Rabbit polyclonal anti-Glial Fibrillary Acidic Protein recognizes human, mouse, and rat GFAPs.

Immunogène

synthetic peptide corresponding to an internal C-terminal sequence of human GFAP.

Application

Rabbit polyclonal anti-GFAP antibody is used to tag glial fibrillary acidic protein for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of glial fibrillary acidic protein in cell:cell communications, mitosis and cell structure. Anti-GFAP has proven a valuable tool for use in immunocytochemical localization of GFAP in normal central nervous system tissue, certain tumors and metastases of the glial antigen, as well as for immunofluorescent labeling of cultured mammalian cells.

Forme physique

solution in phosphate buffered saline, containing 0.02% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Christopher G Dengler et al.
Scientific reports, 7, 42090-42090 (2017-02-22)
In a mouse model of temporal lobe epilepsy, multicellular calcium imaging revealed that disease emergence was accompanied by massive amplification in the normally sparse, afferent stimulation-induced activation of hippocampal dentate granule cells. Patch recordings demonstrated reductions in local inhibitory function
Jesús Agulla et al.
Theranostics, 4(1), 90-105 (2014-01-08)
The use of theranostics in neurosciences has been rare to date because of the limitations imposed on the free delivery of substances to the brain by the blood-brain barrier. Here we report the development of a theranostic system for the
Badrah Alghamdi et al.
Frontiers in neuroanatomy, 9, 49-49 (2015-06-25)
The extent to which NG-2(+) cells form a distinct population separate from astrocytes is central to understanding whether this important cell class is wholly an oligodendrocyte precursor cell (OPC) or has additional functions akin to those classically ascribed to astrocytes.
Maha Saber et al.
Journal of neurotrauma, 34(2), 423-435 (2016-03-16)
Traumatic brain injury (TBI) affects 1.7 million persons annually in the United States (Centers for Disease Control and Prevention). There is increasing evidence that persons exposed to TBI have increased risk of the development of multiple neurodegenerative conditions, including Alzheimer
G F Hamilton et al.
Neuroscience, 265, 274-290 (2014-02-12)
Neonatal alcohol exposure impairs cognition and learning in adulthood and permanently damages the hippocampus. Wheel running (WR) improves hippocampus-associated learning and memory and increases the genesis and survival of newly generated neurons in the hippocampal dentate gyrus. WR significantly increases
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique