A search of the website does not show a density for product G4155. For products supplied in small quantities, it is not common for the density to be measured. However, for solutions available in larger quantities, density information is often provided. Although the density for G4155 is not stated, there is some useful information available from the website. The physical form of the product is a suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, and 0.5 mM mercaptoethanol at pH 7.4.
G4155
β-Galactosidase from Escherichia coli
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Synonyme(s) :
β-D-Galactoside galactohydrolase, Lactase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Forme
aqueous glycerol suspension
Niveau de qualité
Activité spécifique
≥500 units/mg protein (biuret)
Poids mol.
465 kDa
Température de stockage
−20°C
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Catégories apparentées
Application
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Actions biochimiques/physiologiques
βLa β-galactosidase clive le lactose en ses constituants monosaccharides, le glucose et le galactose. Elle catalyse également la transglycosylation du glucose en allolactose, inducteur de la β-galactosidase, dans une boucle de rétroaction.
Propriétés physiques
Poids moléculaire du tétramère : 465 kDa (chaque sous-unité ayant 116,3 kDa)
Définition de l'unité
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Forme physique
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Autres remarques
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
Inhibiteur
Réf. du produit
Description
Tarif
Substrat
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Les clients ont également consulté
Théophile Tschamber et al.
Bioorganic & medicinal chemistry, 11(17), 3559-3568 (2003-08-07)
The syntheses of four glyco-imidazoles, which are pentose-derivatives belonging to the D-series, as well as the syntheses of their L-enantiomers, are reported. Starting from the known linear xylo, lyxo, arabino, and ribo imidazolo-pentoses in both the L- and the D-series
Olga Ermakova et al.
Brain sciences, 11(6) (2021-07-03)
Acquisition of detailed anatomical and molecular knowledge from intact biological samples while preserving their native three-dimensional structure is still a challenging issue for imaging studies aiming to unravel a system's functions. Three-dimensional micro-CT X-ray imaging with a high spatial resolution
Christophe Laumonnerie et al.
Development (Cambridge, England), 141(3), 594-603 (2014-01-23)
The establishment of anatomically stereotyped axonal projections is fundamental to neuronal function. While most neurons project their axons within the central nervous system (CNS), only axons of centrally born motoneurons and peripherally born sensory neurons link the CNS and peripheral
H Morreau et al.
The Journal of biological chemistry, 264(34), 20655-20663 (1989-12-05)
We have isolated two cDNAs encoding human lysosomal beta-galactosidase, the enzyme deficient in GM1-gangliosidosis and Morquio B syndrome, and a beta-galactosidase-related protein. In total RNA from normal fibroblasts a major mRNA of about 2.5 kilobases (kb) is recognized by cDNA
Richard D Bagshaw et al.
Biochimica et biophysica acta, 1588(3), 247-253 (2002-10-24)
Primary deficiency of beta-galactosidase results in GM1 gangliosidosis and Morquio B disease. Of the more than 40 disease-causing mutations described in the Gal gene to date, about 75% are of the missense type and are scattered along the length of
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Could you please advise if the density of the aqueous suspension of beta galactosidase from Escherichia coli (G4155) can be assumed as 1 uL of solution is equal to 1 mg, given that the SDS and provided enzyme assay do not contain this information?
1 answer-
Helpful?
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We recently purchased this enzyme. Information given regarding enzyme volume is only 1000 units. I want to know what would be units per uL. How much is the liquid volume in the given product to calculate the enzyme concentration? Provide proper informatio
1 answer-
Units/ml can be calculated using the numbers reported in batch specific CofA. Below is an example.
CofA of G4155, lot 0000303635
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G4155-3KU0000303635.pdf
1.5 mgP/ml X 766 units/mg Protein = 1149 units/mlHelpful?
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