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Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit

for cellular ATP determination

Synonyme(s) :

ATP bioluminescence assay kit

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32160414
Nomenclature NACRES :
NA.26

Niveau de qualité

Température de stockage

−20°C

Description générale

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit may be employed for the bioluminescent determination of adenosine 5′-triphosphate (ATP) released from a suspension of viable somatic cells. Estimates of cell concentrations may be calculated if it is assumed that the ATP content per viable cell remains constant. The number of viable somatic cells are selectively counted because, as a cell dies, its ATP is rapidly degraded.

Application

The Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit has been used to determine accurately the level of ATP present in neutrophils isolated from the blood of over 100 human test patients.

Composants de kit seuls

Réf. du produit
Description

  • FL-AAB Dilution buffer 1 mL/vial

  • FL-AAM Assay mix 1 mL/vial

  • FL-AAS ATP standard 1 mL/vial

  • FL-SAR Somatic cell releasing reagent 1 mL/vial

Pictogrammes

CorrosionEnvironment

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Code de la classe de stockage

10 - Combustible liquids


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Y Zhao et al.
Redox biology, 12, 129-138 (2017-02-24)
The signaling of reactive oxygen species (ROS) is essential for the maintenance of normal cellular function. However, whether and how ROS regulate stem cells are unclear. Here, we demonstrate that, in transgenic mice expressing the human manganese superoxide dismutase (MnSOD)
Linqing Zhen et al.
Reproductive toxicology (Elmsford, N.Y.), 59, 66-79 (2015-11-20)
Hexavalent chromium reportedly induces reproductive toxicity and further inhibits male fertility in mammals. In this study, we investigated the molecular mechanism by which hexavalent chromium affects motility signaling in boar spermatozoa in vitro. The results indicated that Cr(VI) decreased sperm
Sarah Myhill et al.
International journal of clinical and experimental medicine, 2(1), 1-16 (2009-05-14)
This study aims to improve the health of patients suffering from chronic fatigue syndrome (CFS) by interventions based on the biochemistry of the illness, specifically the function of mitochondria in producing ATP (adenosine triphosphate), the energy currency for all body
Lirui Wang et al.
Reproductive toxicology (Elmsford, N.Y.), 75, 23-32 (2017-11-22)
Cadmium (Cd) has been reported to inhibit mouse sperm motility by inducing the tyrosine phosphorylation of dihydrolipoamide dehydrogenase (DLD). This study aimed to assess the potential effects of vitamin C (Vc) on ameliorating Cd-induced tyrosine phosphorylation of DLD and the
Qicheng Ni et al.
Nature communications, 8, 15755-15755 (2017-06-10)
Diabetes is associated with beta cell mass loss and islet dysfunctions. mTORC1 regulates beta cell survival, proliferation and function in physiological and pathological conditions, such as pregnancy and pancreatectomy. Here we show that deletion of Raptor, which is an essential

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