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Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) assay mix

lyophilized powder

Synonyme(s) :

ATP assay kit, ATP detection kit

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32160414
Nomenclature NACRES :
NA.84

Forme

lyophilized powder

Niveau de qualité

Utilisation

1 vial sufficient for ≥50 assays

Technique(s)

PCR: suitable

Température de stockage

−20°C

Description générale

The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.

Application

Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.
The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes.

Composants

Contains luciferase, luciferin, MgSO4, EDTA, DTT, BSA, and Tricine buffer.

Reconstitution

Reconstitute with 5 ml of sterile water.

Pictogrammes

Corrosion

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Eye Dam. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

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Buse Cevatemre et al.
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C J Williams et al.
Osteoarthritis and cartilage, 26(6), 797-806 (2018-03-27)
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A trans-platinum (II) complex induces apoptosis in cancer stem cells of breast cancer
Aztopal N, et al.
Bioorganic & Medicinal Chemistry, 25(1), 269-276 (2017)
Guiping Sui et al.
American journal of physiology. Renal physiology, 306(3), F286-F298 (2013-11-29)
The urothelium is a newly recognized sensory structure that detects bladder fullness. Pivotal to this sensory role is the release of ATP from the urothelium. However, the routes for urothelial ATP release, its modulation by receptor-mediated pathways, and the autocrine/paracrine
Anita Jemec et al.
Environment international, 87, 20-32 (2015-12-08)
Within the FP7 EU project NanoValid a consortium of six partners jointly investigated the hazard of silver nanoparticles (AgNPs) paying special attention to methodical aspects that are important for providing high-quality ecotoxicity data. Laboratories were supplied with the same original

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