F2047
Anticorps anti-c-Myc
clone 9E10, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Anti-c-Myc
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.56
Produits recommandés
Source biologique
mouse
Conjugué
FITC conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
9E10, monoclonal
Forme
buffered aqueous solution
Espèces réactives
human
Conditions de stockage
protect from light
Validation améliorée
recombinant expression
Learn more about Antibody Enhanced Validation
Concentration
5 μg/mL (suitable for immunoflourescence)
Technique(s)
immunocytochemistry: 5.0 μg/mL using mammalian cells transfected with c-Myc tagged fusion protein, fixed with paraformaldehyde.
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MYC(4609)
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Description générale
Monoclonal Anti-c-Myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice. The antibody is isolated from ascites fluid and conjugated to fluorescein isothiocyanate isomer I (FITC). Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein. c-Myc is a proto-oncogene localized on long arm of human chromosome 8. The encoded protein contains an N-terminal transactivation domain.
Spécificité
Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein.
Immunogène
peptide synthétique de la protéine p62c-Myc humaine.
Application
Monoclonal Anti-c-Myc−FITC antibody produced in mouse has been used in immunofluorescence microscopy and immunocytochemistry.
Actions biochimiques/physiologiques
c-Myc plays a vital role in regulation of cell growth, proliferation, differentiation and apoptosis. It is implicated in DNA replication, RNA splicing or transcription. Deregulation in the gene is associated with the development of various types of human cancers. An epitope located within amino acids 410-419, containing the sequence EQKLISEEDL of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tag fusion proteins. Epitope tags provide a method to localize gene products in a variety of cell types, to study the topology of proteins and protein complexes, and to identify associated proteins. In addition, it allows characterization of newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in “frost-free” freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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