E1257

Facteur de croissance épidermique from murine submaxillary gland

lyophilized powder, BioReagent, suitable for cell culture

• Synonyme(s) : EGF, Facteur de croissance cellulaire épidermique
• Numéro CAS: 62229-50-9
• Numéro CE : 263-468-7
• Numéro MDL: MFCD01634807
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: mouse submaxillary glands
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Pureté: ≥98% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 0.02-2.0 ng/mL EC₅₀
• Conditionnement: pkg of 0.1 mg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Couleur: white
• Numéro d'accès UniProt: P01132
• Température de stockage: 2-8°C
• Informations sur le gène: mouse ... Egf(13645)

Description

Description générale

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor. It is majorly expressed in kidney, parotid gland and salivary gland.

Application

Epidermal Growth Factor from murine submaxillary gland has been used

• in studying its effects on the development of caustic esophageal stricture in rats
• for preparation of MCF-10A cell line
• for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)

Actions biochimiques/physiologiques

Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation. EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia. EGF plays a major role in the maintenance of hair and skin development.

Forme physique

Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.

Notes préparatoires

Purified by gel filtration and ion-exchange chromatography.

Remarque sur l'analyse

The biological activity is measured in a proliferation assay using BALB/MK cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)