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DUO92021

Sigma-Aldrich

Duolink® In Situ PLA® Probe Anti-Human MINUS

Affinity purified Donkey anti-Human IgG (H+L)

Synonyme(s) :

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent

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About This Item

Code UNSPSC :
41105331
Nomenclature NACRES :
NA.41

Source biologique

donkey (Polyclonal)

Forme d'anticorps

affinity purified immunoglobulin (secondary antibody)

Gamme de produits

Duolink®

Espèces réactives

human

Technique(s)

immunofluorescence: suitable
proximity ligation assay: suitable

Adéquation

suitable for brightfield
suitable for fluorescence

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Application

Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting and more.

To perform a complete Duolink® PLA® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment. HRP is also available for brightfield detection.
Specificity

PLA probe anti-Human reacts with whole molecule human IgG and the light chains of other human immunoglobulins. The PLA Probe Anti-Human Minus has minimal cross-reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, mouse, rabbit, rat, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

Application Note

Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

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Caractéristiques et avantages

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Composants

This product is comprised of the following:
  • 5x PLA Probe Anti-Human MINUS - Donkey anti-human secondary antibody conjugated to oligonucleotide MINUS
  • 1x Blocking Solution - Reagent for blocking of the sample
  • 1x Antibody Diluent - For dilution of PLA probes and primary antibodies

See datasheet for more information.

Informations légales

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H317 - H411

Classification des risques

Aquatic Chronic 2 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Juan J Ferreira et al.
iScience, 24(11), 103210-103210 (2021-11-09)
Depolarization of the myometrial smooth muscle cell (MSMC) resting membrane potential is necessary for the uterus to transition from a quiescent state to a contractile state. The molecular mechanisms involved in this transition are not completely understood. Here, we report

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Duolink® PLA Troubleshooting Guide

Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

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Things to consider for preparation, setup and execution of the Duolink® assay protocol

Contenu apparenté

Duolink® PLA Applications

Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

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