Hepa 1-6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92110305, mouse liver, Not specified

• Synonyme(s) : Hepa 1-6 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse liver
• Conditionnement: tube of 5 μg 92110305-DNA-5UG; pkg of vial of cells 92110305-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Albumin, alpha-foetoprotein, alpha 1-antitrypsin, amylase
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse hepatoma

Description de la lignée cellulaire

Derived from BW7756 tumour in a C57L mouse. Secretes several liver products including albumin, α-fetoprotein, α 1-antitrypin and amylase.

Application

Hepa 1-6 has been used to study the biodistribution of polymer hydrogel capsules for the delivery of therapeutics. It has also been used to evaluate the potential of interferon regulatory factor-1 (IRF-1) for cytokine gene therapy of hepatocellular carcinoma (HCC).

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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