IMR 32

86041809, human nerve, Fibroblast/neuroblast

• Synonyme(s) : IMR32 Cells, Institute for Medical Research-32 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: IMR 32, 86041809
• Source biologique: human nerve
• Mode de croissance: Adherent
• Caryotype: 2n = 46
• Morphologie: Fibroblast/neuroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian neuroblastoma

Description de la lignée cellulaire

Established from an abdominal mass occurring in a 13 month old Caucasian male. The tumour was diagnosed as a neuroblastoma with two rare areas of orgonoid differentiation. The culture is a mixture of two morphologically distinct cell types, a small neuroblast-like cell and the other a large hyaline fibroblast. IMR 32 is partially resistant to polioviruses type 3 and highly resistant to ECHO-II. It is susceptible to Coxsackie B3.

Application

IMR 32 has been used to study the transmigration of cells through a human in vitro blood-cerebrospinal fluid barrier (BCSFB) model.

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. The cells have a low plating efficiency and cell attachment can take up to 2-4 days after resuscitation. Cells easily detach. May n

Autres remarques

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