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N4888

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

Ham’s F-12

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Nutrient Mixture F-12 Ham, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Niveau de qualité

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Nutrient Mixture F-12 is suitable for the growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using Chinese hamster ovary (CHO) cells.

Application

Nutrient Mixture F-12 Ham has been used:
  • in Minimum Essential Medium to maintain SH-SY5Y human neuroblastoma cells
  • to culture female-originated neuroblastoma cells
  • to culture primary retinal pigment epithelium (RPE) cells and RPE cell lines

Reconstitution

Supplement with 0.146 g/L L-glutamine.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Kambiz Shekdar et al.
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Roger Oria et al.
Nature, 552(7684), 219-224 (2017-12-07)
Cells can sense the density and distribution of extracellular matrix (ECM) molecules by means of individual integrin proteins and larger, integrin-containing adhesion complexes within the cell membrane. This spatial sensing drives cellular activity in a variety of normal and pathological
Chanan Meydan et al.
Frontiers in immunology, 11, 590870-590870 (2020-11-10)
The COVID-19 pandemic exerts inflammation-related parasympathetic complications and post-infection manifestations with major inter-individual variability. To seek the corresponding transcriptomic origins for the impact of COVID-19 infection and its aftermath consequences, we sought the relevance of long and short non-coding RNAs
Chiara Vidoni et al.
Neurochemistry international, 117, 174-187 (2017-05-24)
Parkinsonian-like motor deficits in Huntington's Disease (HD) patients are associated with abnormal dopamine neurotransmission in the striatum. Dopamine metabolism leads to the formation of oxidized dopamine quinones that exacerbates mitochondrial dysfunction with production of reactive oxygen species (ROS) that eventually

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