Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
J774.2 Cell Line from mouse
85011428, mouse blood
Synonyme(s) :
J774.2 Cells
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About This Item
Code UNSPSC :
41106514
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Nom du produit
J774.2 Cell Line from mouse, BALB/C monocyte macrophage, 85011428
Source biologique
mouse blood
Mode de croissance
Semi-adherent
Caryotype
Not specified
Produits
Not specified
Récepteurs
Immunoglobulin, complement
Technique(s)
cell culture | mammalian: suitable
Maladie(s) pertinente(s)
cancer
Conditions d'expédition
dry ice
Température de stockage
−196°C
Origine de la lignée cellulaire
Mouse BALB/c monocyte macrophage
Description de la lignée cellulaire
Recloned from J774.1 original ascites and solid tumour. Produces IL-1. Cell line J774.2 was recloned from the original ascites and solid tumor J774.1, and produces interleukin-1 (IL-1).
Application
J774.2 Cell Line from mouse has been used:
- to study the effect of the growth hormone secretagogue peptide A233 on J774.2 cell line[1]
- to study the ability of Mycobacterium tuberculosis clinical isolates to penetrate and grow inside the J774.2 cell line[2]
- to study the effect of ivy leaf dry extract EA 575 on the lipopolysaccharide-induced release of interleukin-6 (IL-6) from J774.2 cell line[3]
Milieu de culture
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Procédure de repiquage
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are semi-adherent i.e. some cells grow in suspension , some loosely attach to the surface and others flattened out and attach to the flask. They should be passaged by scraping. Cells should not be allowed to overgrow and become confluent as this can lead to a loss of the flattened adherent cell characteristic.
Autres remarques
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How many Cell Line cells are in the vial?
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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Are my Cell Line cells mycoplasma tested?
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ECACC routinely tests all manufactured cell banks for mycoplasma.
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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What medium should I use when I thaw the Cell Line cells?
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The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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