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C9658

Sigma-Aldrich

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Synonyme(s) :

γ-Glutamyl hydrolase

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About This Item

Numéro CAS:
9074-87-7
Numéro de classification (Commission des enzymes):
3.4.17.11 (BRENDA, IUBMB)
Numéro CE :
232-978-1
Numéro MDL:
MFCD00130719
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

300

Activité spécifique

≥3 units/mg protein

Poids mol.

homodimer ~90 kDa

Composition

Protein, ~70% biuret

Solubilité

H2O: soluble 0.5 mg/mL

Température de stockage

−20°C

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Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Actions biochimiques/physiologiques

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

Forme physique

Contains sodium acetate salt.

Notes préparatoires

Chromatographically purified
Solutions should be prepared fresh prior to use.

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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The enzymatic hydrolysis of methotrexate and folic acid.
C C Levy et al.
The Journal of biological chemistry, 242(12), 2933-2938 (1967-06-25)
Edgar M Pera et al.
Gene expression patterns : GEP, 3(2), 147-152 (2003-04-25)
Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel
Jeremy P Mallari et al.
Bioorganic & medicinal chemistry, 12(22), 6011-6020 (2004-10-23)
A series of alkyl and aryl phosphonyl, thiophosphonyl, and dithiophosphonyl derivatives of (S)- and (R)-glutamic acid were prepared and examined for inhibitory potency against glutamate carboxypeptidase (carboxypeptidase G). The acquisition of the phosphonamidodithioic acids and the individual phosphonamidothioic acid diastereomers
S G Sligar et al.
Proceedings of the National Academy of Sciences of the United States of America, 71(10), 3906-3910 (1974-10-01)
Methylene hydroxylation by cytochrome P-450(cam) (cytochrome m) can be resolved into four distinct steps: substrate addition, m(o) --> m(os); reduction, m(os) --> m(rs); dioxygen addition, m(rs) --> m(O2) (rs); followed by a second putidaredoxin (Pseudomonas putida ferredoxin)-mediated reduction and product
Hiroki Nagai et al.
International journal of oncology, 41(1), 24-30 (2012-05-04)
Pemetrexed (PEM) is a novel, multitargeted, antifolate, antineoplastic agent for the treatment of non-small cell lung cancer and malignant pleural mesothelioma. Additional effects of nitric oxide (NO) donors on the chemosensitivity of cancers have been reported. However, the effects of
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