C9358
Anti-Chk1 antibody, Mouse monoclonal
~2 mg/mL, clone DCS-310, purified from hybridoma cell culture
Synonyme(s) :
Anti-CHK1
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
DCS-310, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen 54 kDa
Espèces réactives
human
Concentration
~2 mg/mL
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells
Isotype
IgG2b
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CHEK1(1111)
Description générale
Checkpoint kinase 1 (Chk1) protein is a serine/threonine-protein kinase with a molar mass of 54 kDa. Monoclonal Anti-Chk1 (mouse IgG2b isotype) is derived from the DCS-310 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human Chk1.
Immunogène
recombinant human Chk1.
Application
Monoclonal Anti-Chk1 antibody produced in mouse has been used in:
- immunoprecipitation
- immunohistochemistry
- immunoblotting
Monoclonal Anti-Chk1 antibody produced in mouse is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections), immunoprecipitation, microarray and western blot at a working concentration of 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells. It was used for immunohistochemistry in a study to investigate the specific role of Chk1 phosphorylations in cell survival and checkpoint activation. It was used for immunohistochemistry at a working dilution of 1:4000 in a study to explore Chk1 knockdown as a novel therapeutic approach to arrest cell-cycle progression in MM cells, thus increasing the rate of cell death. It was used for the immunoprecipitation and detection of total Chk1 by western blot to study a possible regulatory relationship between Chk1 and CK1, a member of the casein kinase 1 family.
Actions biochimiques/physiologiques
Checkpoint kinase 1 (Chk1) protein acts as a cell cycle checkpoint regulator.
The protein propagates signals from damaged or unreplicated DNA. It is expressed and active only in S-G2 phases of the cell cycle. In response to DNA damage, it phosphorylates CDC25C at Ser 216, thus preventing activation of the Cdc2-cyclin B complex and mitotic entry, blocking cell cycle progression. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Chk1 can phosphorylate Wee1, a negative regulator of G2 to M transition.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
C Lukas et al.
Cancer research, 61(13), 4990-4993 (2001-06-30)
The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase
Solange Romagnoli et al.
The American journal of pathology, 174(3), 762-770 (2009-02-17)
Cell-cycle defects are responsible for cancer onset and growth. We studied the expression profile of 60 genes involved in cell cycle in a series of malignant mesotheliomas (MMs), normal pleural tissues, and MM cell cultures using a quantitative polymerase chain
Identification of potential therapeutic targets in malignant mesothelioma using cell-cycle gene expression analysis
Romagnoli S, et al.
The American Journal of Pathology, 174(3), 762-770 (2009)
Hironori Abe et al.
Human molecular genetics, 27(7), 1136-1149 (2018-01-24)
The continuity of life depends on mechanisms in the germline that ensure the integrity of the genome. The DNA damage response/checkpoint kinases ATM and ATR are essential signaling factors in the germline. However, it remains unknown how a downstream transducer
Specific role of Chk1 phosphorylations in cell survival and checkpoint activation
Niida H, et al.
Molecular and Cellular Biology, 27(7), 2572-2581 (2007)
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