C6974

Anti-CaM Kinase IIα (CaMKIIα) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Numéro MDL: MFCD02095961
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 50 kDa
• Espèces réactives: human, chicken, rat, mouse
• Conditionnement: antibody small pack of 25 μL
• Technique(s): microarray: suitable; western blot: 1:5,000 using synaptosomal fraction of rat brain
• Numéro d'accès UniProt: Q9UQM7
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CAMK2A(815) ; mouse ... Camk2a(12322) ; rat ... Camk2a(25400)

Description

Description générale

Ca²⁺/Calmodulin dependent protein kinase II (CaMKII) belongs to the family of Ser/Thr protein kinases including CaMKI and CaMKIV. CaM kinase II is one of the most abundant protein kinases in the mammalian brain, with the highest expression in neurons of the hippocampus and the cerebral cortex. CaMKII consists of a family of four related isoforms CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK II α and β isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMKII γ and δ isoforms are expressed in all tissues. CaMKII contains a catalytic and regulatory domain. The regulatory domain consists of an autoinhibitory and calmodulin binding site.

Spécificité

The antibody does not cross-react with CaMKIIβ, γ and δ isoforms (60 kDa). Anti-CaM Kinase IIα specifically binds to CaM Kinase IIα (50kDa) in human, mouse, rat and chicken. CaM Kinase IIα immunizing peptide specifically inhibits the staining of CaM Kinase IIα in immunoblotting.

Immunogène

Peptide corresponding to amino acids 461-478 of rat CaM Kinase Iiα, conjugated to KLH. This sequence is identical in human, mouse and chicken CaMKIIα has limited homology (50-60%) with CaM Kinase IIβ, γ and δ subunits.

Application

Anti-CaM Kinase IIα (CaMKIIα) antibody has been used:

• in immunolabelling
• in western blotting
• in immunoprecipitation
• in immunoblotting

Actions biochimiques/physiologiques

CaMKII is involved in many cellular functions in response to Ca²⁺ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation (LTP) and spatial learning, receptor function and regulation of gene expression. CaMKII plays a critical role in LTP, a cellular model of learning and memory. In the CNS, postsynaptic Ca²⁺ influx triggers rapid autophosphorylation and stable activation of CaMKII in a Ca²⁺/calmodulin dependent manner at a threonine residue in the autoinhibitory domain. Autophosphorylation of CaMKIIα at Thr²⁸⁶ has been shown to be required for LTP and learning.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)